Ethnobotanical Insights: Phytochemical Richness, Nutritional Potential, and Bioactivity Evaluation of Bambusa arundinacea and Bambusa nutans
Abstract
Bamboo species are widely utilized in traditional medicine, yet their pharmacological and nutritional potential remains insufficiently explored. This study specifically investigates the ethnobotanical uses, phytochemical composition, nutritional profile, and bioactivities of Bambusa arundinacea and Bambusa nutans from Odisha, India. Ethnobotanical surveys using structured interviews confirmed their application in treating gastrointestinal disorders, wound healing, and infectious diseases. Phytochemical screening revealed the presence of phenolics, flavonoids, tannins, alkaloids, and saponins, with B. arundinacea exhibiting comparatively higher phenolic and flavonoid contents. Nutritional analysis demonstrated appreciable levels of protein, crude fiber, carbohydrates, and essential minerals, highlighting their nutraceutical value. Bioactivity assays showed strong antioxidant activity (DPPH), significant antibacterial effects against Salmonella typhi, and pronounced anthelmintic activity, with B. arundinacea producing shorter paralysis and death times than B. nutans. The Brine Shrimp Lethality Assay further indicated notable cytotoxicity, suggesting the presence of pharmacologically active compounds. Overall, B. arundinacea displayed comparatively superior bioactivity and phytochemical richness, supporting traditional medicinal claims and establishing both species as promising sources of antioxidant, antimicrobial, and antiparasitic agents, thereby warranting further isolation and characterization of active constituents.
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Aisha Anjum, A., Tabassum, K., & Ambar, S. T. (2019). a plant origin drug of Unani medicine–a review. J Ayurvedic Herb Med, 5, 31-4.https://doi.org/10.31254/jahm.2019.5109
Ajaiyeoba, E. O., Onocha, P. A., &Olarenwaju, O. T. (2001). In vitro anthelmintic properties of Buchholziacoriaceae and Gynandropsisgynandra extracts. Pharmaceutical biology, 39(3), 217-220. https://doi.org/10.1076/phbi.39.3.217.5936
Ali, N. M., Annegowda, H. V., Mansor, S. M., Ismail, S., Ramanathan, S., & Mordi, M. N. (2012). Phytochemical screening, antioxidant and analgesic activities of Croton argyratus ethanolic extracts. Journal of medicinal plants research, 6(21), 3724-3731.https://doi.org/10.5897/JMPR11.743
Avilés-Betanzos, K. A., Oney-Montalvo, J. E., Cauich-Rodríguez, J. V., González-Ávila, M., Scampicchio, M., Morozova, K., ... & Rodríguez-Buenfil, I. M. (2022). Antioxidant capacity, vitamin C and polyphenol profile evaluation of a capsicum chinense by-product extract obtained by ultrasound using eutectic solvent. Plants, 11(15), 2060.https://doi.org/10.3390/plants11152060
Azzahra, V C., Mardiana, S., Suharyadi, S., Sianipar, R., & Ramadhan, D S. (2025). Effect of Solvent Polarity on Extraction Yield, Phytochemical Composition, and Antioxidant Activity of Curcuma xanthorrhiza Roxb. and Moringa oleifera Lam. Biology Medicine & Natural Product Chemistry.14(2):1273-1284. https://doi.org/10.14421/biomedich.2025.142.1273-1284
Babbar, N., Oberoi, H. S., Sandhu, S. K., & Bhargav, V. K. (2014). Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants. Journal of food science and technology, 51(10), 2568-2575.https://doi.org/10.1007/s13197-012-0754-4
Bajwa, H. K., Santosh, O., &Chongtham, N. (2020). Bioactive compounds in bamboo shoot. In Bioactive compounds in underutilized vegetables and legumes (pp. 1-22). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-44578-2_24-1
Benjamin, M. A. Z., Saikim, F. H., Ng, S. Y., &Rusdi, N. A. (2023). A comprehensive review of the ethnobotanical, phytochemical, and pharmacological properties of the genus Bambusa. Journal of Applied Pharmaceutical Science, 13(5), 001-022. https://dx.doi.org/10.7324/JAPS.2023.98082
Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917. https://doi.org/10.1139/o59-099
Cao, G., Sofic, E., & Prior, R. L. (1997). Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships. Free Radical Biology and Medicine, 22(5), 749-760. https://doi.org/10.1016/S0891-5849(96)00351-6
Chanda, S. V., &Nagani, K. V. (2010). Antioxidant capacity of Manilkara zapota L. leaves extracts evaluated by four in vitro methods. Nature and Science, 8(10), 260-266.
Chitiva, L. C., Rezende-Teixeira, P., Leão, T. F., Lozano-Puentes, H. S., Londoño, X., Díaz-Ariza, L. A., ... & Castro-Gamboa, I. (2024). Metabolomic profiling of Guadua species and its correlation with antioxidant and cytotoxic activities. ACS omega, 9(35), 36939-36960.https://doi.org/10.1021/acsomega.3c09114
Choi, M. H., Yang, S. H., Kim, N. D., & Shin, H. J. (2022). Nomilin from Yuzu Seed Has in vitro antioxidant activity and downregulates melanogenesis in B16F10 melanoma cells through the PKA/CREB signalling pathway. Antioxidants, 11(9), 1636. https://doi.org/10.3390/antiox11091636
Devi, R. S., Satapathy, K. B., & Kumar, S. (2023). Validation of tribal claims for formulation of future drugs through evaluation of ethno-pharmacological values of Ludwigiaadscendens. Medicinal plants, 15(4), 691-697. https://doi.org/10.5958/0975-6892.2023.00069.2
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350-356. https://doi.org/10.1021/ac60111a017
Fitri, A., Asra, R., &Rivai, H. (2020). Overview of the traditional, phytochemical, and pharmacological uses of gold bamboo (Bambusa vulgaris). World Journal of Pharmacy and Pharmaceutical Sciences, 9(8), 299-318.https://doi.org/10.20959/wjpps20208-16877
Gagliano, J., Anselmo-Moreira, F., Sala-Carvalho, W. R., & Furlan, C. M. (2022). What is known about the medicinal potential of Bamboo?. Advances in Traditional Medicine, 22(3), 467-495. https://doi.org/10.1007/s13596-020-00536-5
Ghaffar, N., & Perveen, A. (2025). Solvent polarity effects on extraction yield, phenolic content, and antioxidant properties of Malvaceae family seeds: a comparative study. New Zealand Journal of Botany, 63(4), 627-637.https://doi.org/10.1080/0028825X.2024.2392705
Giri Singh, S. (2013). Nutritional value of bamboo shoots of Manipur a North East State of India. Life Science Bulletin, 10(2), 325–328. https://connectjournals.com/file_full_text/2033302H_325-328.pdf
Goswami, A., Bhattacharjee, R., & Gorai, S. (2024). Phytochemical and pharmacological insights into bamboo: A comprehensive review of its medicinal value. International Research Journal of Modernization in Engineering Technology and Science, 6(10), 3056–3064. https://doi.org/10.56726/IRJMETS62694
Gouda, S., Moharana, R. R., Das, G., & Patra, J. K. (2013). Free radical scavenging potential of extracts of Gracilaria verrucosa (L) (Harvey): An economically important seaweed from Chilika lake, India. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), 707-710. https://innovareacademics.in/journal/ijpps/Vol6Issue1/8348.pdf
Harborne, A. J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. Springer science & business media. https://link.springer.com/book/9780412572609
Jena, N., Rout, S., Devi, R. S., & Kumar, S. (2024). Phytochemical and cytotoxicity analysis of fruits of Zanthoxylum asiaticum (L.) Appelhans, Groppo& J. Wen: A minor fruit plant of Odisha, India. E-planet, 22(1), 62-70. https://e-planet.co.in/wp-content/uploads/2024/07/Phytochemical.pdf
Jena, N., Rout, S., Mishra, S., & Kumar, S. (2025). Evaluation of quantitative ethnobotanical uses in Mayurbhanj district, Odisha, India. Journal of Biodiversity and Conservation, 9(1), 1-29. https://doi.org/10.5281/zenodo.14674405
Jena, N., Vimala, S. B., Patra, A., Sharma, B. P., Hossain, E., & Kumar, S. (2025). Methods for ethnobotanical data collection, phytochemistry, antioxidant, anthelmintic, and antimicrobial activities for pharmacological evaluation of medicinal plants. Journal of Biodiversity and Conservation, 9(2), 87-107. https://doi.org/10.5281/zenodo.15368734
Joshi, B., Indira, A., Kalyan, N., Chongtham, N., & Koul, A. (2026). Ethnobotanical and Pharmacological Insights into Bambusa Genus. In Exploring Medicinal Bamboos (pp. 152-165). CRC Press. https://doi.org/10.1201/9781003485933-11
Jyotirmayee, B., & Mahalik, G. (2022). Traditional uses and variation in curcumin content in varieties of curcuma–the saffron of India. Ambient Science, 9(1), 06-12. https://doi.org/10.21276/ambi.2022.09.1.rv01
KC, B. M., Gauchan, D. P., Khanal, S. N., & Lamichhane, J. (2024). Phytochemical screening, antioxidant and antibacterial activity of bamboo leaf collected from agroecosystem of the Central Siwalik region, Nepal. Vegetos, 37(6), 2600-2606.https://doi.org/10.1007/s42535-023-00761-8
Khouri, M. R., Huang, G., & Shiau, Y. F. (1989). Sudan stain of fecal fat; new insight into an old test. Gastroenterology, 96(2), 421-427. https://doi.org/10.1016/0016-5085(89)91566-7
Kimura, I., Kagawa, S., Tsuneki, H., Tanaka, K., & Nagashima, F. (2022). Multitasking bamboo leaf-derived compounds in prevention of infectious, inflammatory, atherosclerotic, metabolic, and neuropsychiatric diseases. Pharmacology & Therapeutics, 235, 108159. https://doi.org/10.1016/j.pharmthera.2022.108159
Krishnaraju, A. V., Rao, T. V., Sundararaju, D., Vanisree, M., Tsay, H. S., &Subbaraju, G. V. (2005). Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering,3(2), 125-134. https://gigvvy.com/journals/ijase/articles/ijase-200510-3-2-125
Kumar, P. P. S., Kumari, K. U., Devi, M. P., Choudhary, V. K., & Sangeetha, A. (2017). Bamboo shoot as a source of nutraceuticals and bioactive compounds: a review. Indian Journal of Natural Products and Resources, 8(1), 32–46.
Kumar, S. N., Mishra, S., & Kumar, S. (2021). Documentation of indigenous traditional knowledge (ITK) on commonly available plants in Koira range, Bonai Forest Division, Sundargarh, Odisha, India. Asian Plant Research Journal, 8(4), 83-95. https://doi.org/10.9734/APRJ/2021/v8i430188
Kumar, S., Behera, S. P., & Jena, P. K. (2013). Validation of tribal claims on Dioscorea pentaphylla through phytochemical screening and evaluation of antibacterial activity. Plant Science Research, 35(1), 55-61.
Kumar, S., Mahanti, P., Singh, N. R., Rath, S. K., Jena, P. K., & Patra, J. K. (2017). Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India. Brazilian Journal of Pharmaceutical Sciences, 53(4), e17006. https://doi.org/10.1590/s2175-97902017000417006
Kumar, S., Tripathy, P. K., & Jena, P. K. (2012). Ethnobotany and bioactive compounds in leaf of Bixa orellana L. and its toxicity to Artemia salina. Plant Science Research, 34(1&2), 93-96.
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275. https://journalsp.com/wp-content/uploads/2022/10/Word-Template_Sample.pdf
Macwan, C., Patel, H. V., & Kalia, K. (2010). A comparative evaluation of in vitro antioxidant properties of Bamboo Bambusa arundinacea leaves extracts. Journal of cell and tissue Research, 10(3), 2413.ISSN: 0974- 0910
Mansour-Ghanaei, F., Poostizadeh, G., Joukar, F., &Siavoshi, F. (2022). Efficacy of disc diffusion and agar dilution methods in evaluating Helicobacter pylori susceptibility to antibiotics. Middle East Journal of Digestive Diseases, 14(2), 207-213. https://doi.org/10.34172/mejdd.2022.274
Marndi, S., Barik, M., & Kumar, S. (2024). Phytochemical and cytotoxicity analysis of Eulophiaexplanata Lindl. (Tuber extracts). African Journal of Biological Sciences, 6(4), 2751–2756. https://doi.org/10.48047/AFJBS.6.Si4.2024.2751-2756
Muda, I., Prastowo, J., Nurcahyo, W., & Sarmin, S. (2021). Anthelmintic effect of Indigofera tinctoria L on Haemonchuscontortus obtained from sheep in Indonesia. Veterinary world, 14(5), 1272.https://doi.org/10.14202/vetworld.2021.1272-1278
Nahar, A., Khandakar, M. J., Mamun, M. J. I., Rasel, M. H., Ihsan, A. B., Raj, A., ... & Saito, T. (2025). Investigation of Analgesic, Anti-Inflammatory, and Thrombolytic Effects of Methanolic Extract and Its Fractions of Dischidia bengalensis: In Vitro and In Vivo Studies with In Silico Interventions. molecules, 30(18), 3724.https://doi.org/10.3390/molecules30183724
Nazreen, S. (2022). Ethnomedicinal uses, Pharmacological and phytochemical studies of Bambusa arundinaceae (Retz.) Willd. (a review). Oriental Journal of Chemistry, 38(2), 247-258. https://doi.org/10.13005/ojc/380204
Nhamussua, R. L., Mabiki, F. P., Mwakalesi, A. J., & McGaw, L. J. (2026). Screening anticancer activity by Brine shrimp lethality test of extracts of Annona stenophylla (Engl. & Diels), Strophanthus petersianus (Klotzsch) and Synadenium glaucescens (Pax). PLoS One, 21(1), e0336636. https://doi.org/10.1371/journal.pone.0336636
Nirmala, C., Bisht, M. S., & Laishram, M. (2014). Bioactive compounds in bamboo shoots: health benefits and prospects for developing functional foods. International Journal of Food Science and Technology, 49(6), 1425-1431. https://doi.org/10.1111/ijfs.12470
Obadoni, B. O., & Ochuko, P. O. (2002). Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences, 8(2), 203-208. https://doi.org/10.4314/gjpas.v8i2.16033
Owolabi, M. S., &Lajide, L. (2015). Preliminary phytochemical screening and antimicrobial activity of crude extracts of Bambusa vulgaris Schrad. Ex JC Wendl. (Poaceae) from southwestern Nigeria. American Journal of Essential Oils and Natural Products, 3(1), 42-45. https://www.essencejournal.com/pdf/2015/vol3issue1/PartA/2-4-7-449.pdf
Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J. H., ... & Levine, M. (2003). Vitamin C as an antioxidant: evaluation of its role in disease prevention. Journal of the American college of Nutrition, 22(1), 18-35. https://doi.org/10.1080/07315724.2003.10719272
Padmaja, R., Arun, P. C., Prashanth, D., Deepak, M., Amit, A., & Anjana, M. (2002). Brine shrimp lethality bioassay of selected Indian medicinal plants. Fitoterapia, 73(6), 508-510. https://doi.org/10.1016/S0367-326X(02)00182-X
Patel, M., & Mehta, P. (2021). Bamboo a supplement to human health: a comprehensive review on its ethnopharmacology, phytochemistry, and pharmacological activity. The Natural Products Journal, 11(2), 140-168.https://doi.org/10.2174/2210315510666200101092551
Pearson, D. (1976). The chemical analysis of foods (7th ed.). Churchill Livingstone. https://www.cabidigitallibrary.org/doi/full/10.5555/19710403806
Ponphaiboon, J., Krongrawa, W., Aung, W., Chinatangkul, N., Limatvapirat, S., &Limatvapirat, S. (2023). Advances in Natural Product Extraction Techniques, Electrospun Fiber Fabrication, and the Integration of Experimental Design: A Comprehensive Review. Molecules. 28(13):5163. https://doi.org/10.3390/molecules28135163
Prakash, J. T. J., Vijayan, N., &Kumararaman, S. (2008). Growth of tetrakis thiourea potassium iodide as new second order optical material. Crystal Research and Technology: Journal of Experimental and Industrial Crystallography, 43(4), 423-427. https://doi.org/10.1002/crat.200711062
Rana, Z. H., Alam, M. K., & Akhtaruzzaman, M. (2019). Nutritional composition, total phenolic content, antioxidant and α-amylase inhibitory activities of different fractions of selected wild edible plants. Antioxidants, 8(7), 203. https://doi.org/10.3390/antiox8070203
Ranganna, S. (1986). Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw-Hill Education. https://books.google.co.in/books/about/Handbook_of_Analysis_and_Quality_Control.html?id=jQN8Kpj0UOMC&redir_esc=y
S, D. B., K, P. D. L., M, G., S, V. L., Reji, A., & F, K. S. (2024). Comparison of the antibacterial efficacy of bamboo shoot ethanol extract with chlorhexidine mouth rinse against salivary Streptococcus mutans and Lactobacillus acidophilus: an ex vivo study. Cureus, 16(1), e53085. https://doi.org/10.7759/cureus.53085
Sadasivam, S., & Manickam, A. (2009). Biochemical Methods. (3-Ed.) New age international (pvt) Ltd. New Delhi, India. Pp, 7-18.
Sebastian, J., Longvah, T., Subhash, K., &Ananthan, R. (2024). Evaluation of Nutritional and Bioactive Components and Antioxidant Potential of Bamboo Rice (Bambusa arundinacea Willd.) with Pharma-Nutritional Role. Indian Journal of Pharmaceutical Sciences, 86(5).https://doi.org/10.36468/pharmaceutical-sciences.1449
Senguttuvan, J., Paulsamy, S., & Karthika, K. (2014). Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaerisradicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine, 4(Supp 1), S359-S367. https://doi.org/10.12980/APJTB.4.2014C1030
Shirazi, O. U., Khattak, M. M. A. K., Shukri, N. A. M., &Nasyriq, M. N. (2014). Determination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices. Journal of Pharmacognosy and Phytochemistry, 3(3), 104-108. https://www.phytojournal.com/archives/view-pdf/391/29.1
Singhal, P., Satya, S., & Naik, S. N. (2021). Fermented bamboo shoots: A complete nutritional, anti-nutritional and antioxidant profile of the sustainable and functional food to food security. Food chemistry: Molecular sciences, 3, 100041.https://doi.org/10.1016/j.fochms.2021.100041
Sun, S., Yu, Y., Jo, Y., Han, J. H., Xue, Y., Cho, M., ... & Zhuang, S. (2025). Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures. Frontiers in pharmacology, 16, 1615338.16:1615338. https://doi.org/10.3389/fphar.2025.1615338
Tanaka, A., Zhu, Q., Tan, H., Horiba, H., Ohnuki, K., Mori, Y., ... & Shimizu, K. (2014). Biological activities and phytochemical profiles of extracts from different parts of bamboo (Phyllostachys pubescens). Molecules, 19(6), 8238-8260. https://doi.org/10.3390/molecules19068238
Tayung, K., Barik, B. P., Jha, D. K., & Deka, D. C. (2011). Identification and characterization of antimicrobial metabolite from an endophytic fungus, Fusarium solani isolated from bark of Himalayan yew. Mycosphere, 2(3), 203-213. Available at https://www.mycosphere.org/pdf/MC2_3_No2.pdf
Trease, G. E., & Evans, W. C. (2002). Pharmacognosy. London: Saunders Publishers.
Ullah, M. O., Haque, M., Urmi, K. F., Zulfiker, A. H. M., Anita, E. S., Begum, M., & Hamid, K. (2013). Anti–bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 3(1), 1-7. https://doi.org/10.1016/S2221-1691(13)60015-5
Zhang, T., Jia, C., Ran, L., Shi, J., Amarmend, T., & Li, H. (2024). The alleviative effects comparison of four flavonoids from bamboo leaves on ulcerative colitis in an Alzheimer mouse model. CNS Neuroscience & Therapeutics, 30(2), e14620. https://doi.org/10.1111/cns.14620.
Zubair, M., Bibi, Z., Rizwan, K., Rasool, N., Zahoor, A. F., & Riaz, M. (2013). In vitro antimicrobial and haemolytic studies of Bambusa arundinaceae leaves. Journal of Applied Pharmaceutical Science, 3(4), 111-115.https://dx.doi.org/10.7324/JAPS.2013.3420

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