Phytochemical Profiling by Hyphenated Technique and Invitro Enzymatic Anti-Diabetic Activity of Ethanolic Extract of (Oryza sativa. L Indica)

M. V. Kumuthavalli; S. Gokul; T. Keerthana; L. Janarthanan; M. Kumar

doi:10.31632/ijalsr.2025.v08i01.005

M. V. Kumuthavalli Department of Pharmacognosy, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed University), Salem, India https://orcid.org/0000-0001-6675-8883
S. Gokul Department of Pharmacy, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed University), Salem, India.
T. Keerthana Department of Pharmacognosy, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed University), Salem, India https://orcid.org/0009-0002-8519-3595
L. Janarthanan Department of Pharmacognosy, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed University), Salem, India https://orcid.org/0000-0002-5011-9013
M. Kumar Vinayaka Mission’s College of Pharmacy, Vinayaka Mission’s Research Foundation (Deemed University), Salem, India https://orcid.org/0000-0002-3513-2383

DOI https://doi.org/10.31632/ijalsr.2025.v08i01.005

Abstract

Diabetes mellitus is one of the metabolic disorders caused by insufficient secretion of insulin from pancreas this leads to increase level of blood glucose level. This causes severe side effects like retinopathy, neuropathy and so on. In India the common food is rice (Oryza sativa) for their day-to-day life which is having excessive carbohydrate and less fibre content. Hence, there is a chance of increased glycemic index level in our body. To overcome this problem there is an alternative food black rice (Oryza sativa. L Indica) contains more fibre and less carbohydrate when compared to white rice. In this research we focussed the phytochemical analysis and invitro pharmacological evaluation by using enzymatic assay method with the use of ethanolic extract of Oryza sativa. L Indica by cold maceration process. Phytochemical analysis of extract revealed that presence of various phytoconstituents like flavonoids, saponins, alkaloids, tannins, phenols and glycosides. In Aldose reductace inhibition assay extract showed significant inhibitory property with and IC50 value of 238.7μg/ml. Results of α- glucosidase inhibitory assay showed IC50 value of 383.2μg/ml and IC50 of 228.6 2μg/ml for α- amylase inhibition activity.The anti-diabetic portential may be due to the presence of flavonoids and phenols present in the extract. In these research it was proved through invitro anti-diabetic potential using enzymatic inhibition activity. In future, through invivo method, the same activity will carried out for further research development towards diabetes. By concluding that black rice can be taken in daily diet by all common people without any complications.

Keywords: Aldose Reductase, Black Rice Diabetes, α-amylase, α- glucosidase

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