The Comparative Study of Physical Exercise Towards Brain Natriuretic Peptide (BNP) and Malondialdehyde (MDA) as Oxidative Stress Markers in Rattus Novergicus Wistar Strain Rats
Abstract
Background: Physical exercise has an effect on biological functions, namely a positive effect in terms of cell repair and a negative effect, namely inhibiting or damaging cells. Strenuous physical exercise can increase muscle injury and oxidative stress in individuals who are not conditioned or are not used to physical exercise. In addition, oxidative stress also plays a role in chronic fatigue syndrome. This occurs because the speed of energy demand exceeds the speed and ability of the oxygen transport system to supply oxygen to the mitochondria. The heart muscle is a muscle that has a major role in physical exercise. Oxidative stress plays an important role in the pathogenesis of myocardial infarction. Objectives: The purpose of this research is to determine the difference between aerobic and anaerobic exercise in the formation of free radicals. The formation of free radicals can be predicted by measuring levels of malondialdehyde (MDA) and Brain Natriuetic Peptide (BNP). Methods: The design used in this study was an experimental study in vivo with a pretest-posttest design with a comparison group. The subjects of this study were 28 male Wistar rats, Rattus novergicus, which were divided into 6 treatment groups and 1 comparison group. The data is taken by doing treadmill physical exercise with a speed of 20m / minute for aerobics and 35m / minute for anaerobics. Different tests of average MDA and BNP levels were used using independent t-tests, ANOVA, and post hoc. Result: The results showed a decrease in MDA and BNP levels in the aerobic treatment group and an increase in anaerobic compared to the comparison group. The analysis showed that there was a significant difference between the aerobic group for 1 day, 3 times a week, and 7 days, respectively, with p 0.05. Conclusion: It can be concluded that aerobic and anaerobic physical exercise performed 1 day, 3 times a week, and 7 consecutive days can increase the antioxidant defence system so that oxidative stress decreases, which is indicated by a decrease in MDA and BNP levels.
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References
American College of Sports Medicine, Thompson, P. D., Franklin, B. A., Balady, G. J., Blair, S. N., Corrado, D., ... & Costa, F. (2007). Exercise and acute cardiovascular events: placing the risks into perspective: a scientific statement from the American Heart Association Council on Nutrition, Physical Activity, and Metabolism and the Council on Clinical Cardiology. Circulation, 115(17), 2358-2368. https://doi.org/10.1161/CIRCULATIONAHA.107.181485
Aristoteles, Shatriadi, H., Zairinayati, Haryoko, I., & Poddar, S. (2021). Comparative study of examination of LED value using the wintrobe method and hemoglobin examination using the Sahli method on rattus norvegicus wistar exposed to cigarette smoke. Malaysian Journal of Medicine and Health Sciences, 17(June), 7–10.
Bordbar, S., Rahimi, E., Ahmadi, N., Bigi, M. A. B., & Aslani, A. (2012). Effect of endurance and strength exercise on release of brain natriuretic peptide. Journal of cardiovascular disease research, 3(1), 22-25. https://doi.org/10.4103/0975-3583.91599
Cowie, M. R., Jourdain, P., Maisel, A., Dahlstrom, U., Follath, F., Isnard, R., ... & Francis, G. (2003). Clinical applications of B-type natriuretic peptide (BNP) testing. European heart journal, 24(19), 1710-1718. https://doi.org/10.1016/S0195-668X(03)00476-7
Ebrahimian, K. (2018). Regulation and Function of MEF2 in Cardiomyocytes [Master's thesis, York University]. Canada. http://hdl.handle.net/10315/35489
El Assar, M., Álvarez-Bustos, A., Sosa, P., Angulo, J., & Rodríguez-Mañas, L. (2022). Effect of physical activity/exercise on oxidative stress and inflammation in muscle and vascular aging. International Journal of Molecular Sciences, 23(15), 8713. 1-36. https://doi.org/10.3390/ ijms23158713
Niessner, A., Ziegler, S., Slany, J., Billensteiner, E., Woloszczuk, W., & Geyer, G. (2003). Increases in plasma levels of atrial and brain natriuretic peptides after running a marathon: are their effects partly counterbalanced by adrenocortical steroids?. European journal of endocrinology, 149(6), 555-559. https://doi.org/10.1530/eje.0.1490555
Nur Wahyu, A. E., Idris, I., Aras, D., Sinrang, A. W., Patellongi, I., & Nawir, N. (2023). Analysis of Changes in Malondialdehyde (MDA) and Lactate Dehydrogenase (LDH) Levels After a 30 km Cycling Event in The Makassar Cycling Community. COMPETITOR: Jurnal Pendidikan Kepelatihan Olahraga, 15(1), 104-115. https://doi.org/10.26858/cjpko.v15i1.43630
Purnomo, M. (2011). Asam Laktat dan Aktivitas SOD Eritrosit pada Fase Pemulihan Setelah Latihan Submaksimal. Jurnal Media Ilmu Keolahragaan Indonesia, 1(2), 155-170. https://doi.org/10.15294/miki.v1i2.2031
Rafaqat, S., & Rafaqat, S. (2023). The role of biomarkers of stress in heart failure. Folia Cardiologica. 19, 1-9. https://doi.org/10.5603/fc.95029
Rahima, R., Flora, R., & Theodorus, T. (2015). Indikator Beban Berlebih Pada Otot Jantung Tikus Wistar Yang Diberi Perlakuan Latihan Fisik Akut. Biomedical Journal of Indonesia, 1(1), 35-39. https://doi.org/10.32539/bji.v1i1.4276
Rahima, R., Flora, R., & Theodorus, T. (2022, December). Increased Secretion of BNP Levels as an Indicator of Overload in the Heart During Physical Exercise Without Rest Days. In 3rd Progress in Social Science, Humanities and Education Research Symposium (PSSHERS 2021) (pp. 364-371). Atlantis Press.
Sagnella, G. A. (2000). Practical implications of current natriuretic peptide research. Journal of the Renin-Angiotensin-Aldosterone System, 1(4), 304–315. https://doi.org/10.3317/jraas.2000.059
Stein, B. C., & Levin, R. I. (1998). Natriuretic peptides: physiology, therapeutic potential, and risk stratification in ischemic heart disease. American heart journal, 135(5), 914-923. https://doi.org/10.1016/S0002-8703(98)70054-7
Wasfy, M. M., & Baggish, A. L. (2016). Exercise dose in clinical practice. Circulation, 133(23), 2297-2313. https://doi.org/10.1161/CIRCULATIONAHA.116.018093
Weeks, B. K., Purvis, M., & Beck, B. R. (2016). Physical activity estimated by the bone-specific physical activity questionnaire is also associated with cardiovascular risk. European journal of sport science, 16(8), 1204-1211. https://doi.org/10.1080/17461391.2016.1153726
Zhou, Z., Chen, C., Teo, E. C., Zhang, Y., Huang, J., Xu, Y., & Gu, Y. (2022). Intracellular oxidative stress induced by physical exercise in adults: systematic review and meta-analysis. Antioxidants, 11(9), 1751. https://doi.org/10.3390/antiox11091751
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