Effect of Vitamin C & E on Enzyme Glutathione Peroxidase Activity (GSH-Px) in Male Wistar Strain Rats Exposed to Carbon Tetrachloride (CCl4)
The free radicals are the cause of cell damage that underlies the emergence of various degenerative diseases. Having an abundant amount of free radicals can cause oxidative stress. Carbon tetrachloride, including chemicals that are metabolized in the liver, its metabolism result is free radical. Its needed to anticipate the damage to the liver. So that, it is needed to have antioxidants from outside to increase the cells defence as neutralizer free radicals like vitamin C and E.
The purpose of this study was to determine the effect of vitamin C and E on glutathione peroxidase enzyme activity on rats exposed to carbon tetrachloride.
The research was conducted experimentally with post test only control group design. The study sample consisted of 25 male wistar strain rats weighing 165-210 g, divided into 5 groups: negative control, positive control (CCl4), treatment I (CCl4 and Vitamin C), treatment II (CCl4 and Vitamin E), treatment III (CCl4, combination of vitamins C and E). CCl₄ was given a single dose of 0.2 mg / kg, the next 24 hours are given Vitamin C 0.8 mg / kg, 0.8 ml vitamin E / kg and a combination of vitamins C 0.8 mg / kg and 0.8 ml/ kg, administered orally for 14 days. Data were analyzed with a confidence level of 95%.
The Results of the study showed the average activity of the enzyme glutathione peroxidase was negative group 114.4 ± 5.5 nmo/ml, positive group 96 ± 7.4 nmo/ml, the first treatment 129.2 ± 5.4 nmo/ml, the second treatment 140.2 ± 3.2nmo/ml, the third treatment 156 ± 4.2 nmo/ml.
It is concluded that vitamin C and E can increase the glutathione peroxidase activity in rats exposed to carbon tetrachloride. It is need to do more research on the effect of vitamin C and E to see the existing network of the liver.
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