Antimicrobial Susceptibility for Bacterial Isolates from Abattoir Effluent of Damaturu-Nigeria

  • Debajyoti Bose Department of Biological Sciences, Yobe State University, K.M. 7 Gujba Road, P.M.B. 1144, Damaturu, Yobe State, Nigeria
  • Muhammad M Kime Department of Biological Sciences, Yobe State University, K.M. 7 Gujba Road, P.M.B. 1144, Damaturu, Yobe State, Nigeria


Hazards caused by effluent into the environment are enormous and may lead to several complications in living forms. Our present research studies were conducted to determine antimicrobial susceptibility and resistance for bacterial isolates collected from abattoirs effluent in Damatruru, Nigeria against commercially available antibiotics. Temperature and pH of the effluent were determined. Bacterial isolates were identified by biochemical method and antimicrobial susceptibility was determined using disc and well diffusion method. The initial temperature of abattoir effluent were 25oC, initial pH of abattoir were 6.7.The bacterial isolates from abattoir effluent were identified as Escherichia coli, Shigella sp. and Staphylococcus aureus. Escherichia coli were resistant to Chloramphenicol(Ch), Sparfloxacin(SP), Ciprofloxacin(CPX), Amoxicillin(AM), Agumentin (AU), Gentamycin(GN), Pefloxacin(PCF) and Streptomycin(S). Shigella sp. showed resistance against Sparfloxacin, Ciprofloxacin, Amoxicillin, Augumentin, Gentamycin, and Streptomycin, whereas Shigella sp.  was sensitive to Septrin (Co-trimoxazole), Chlorophenicol, Pefloxacin and Tarivid (Ofloxacin). The Staphylococcus aureus were resistant to Ampiclox (Ampicillin and Cloxacillin), Zinacef (Cefuroxime), Amoxicillin and Erythromycin, whereas it was sensitive to Pefloxacin, Gentamycin, Ciprofloxacin, Streptomycin and Septrin. For, Well diffusion method, Escherichia coli were resistance to Septrin and Amoxicillin, whereas sensitive to Ciprofloxacin and Tetracycline .Shigella sp. were resistant to Septrin, Amoxicillin whereas, sensitive to Ciprofloxacin and Tetracycline. Staphylococcus aureurs were resistant to Septrin, Amoxicillin. Other showed the susceptibility to antibiotics.

Keywords: Abattoir effluent, Escherichia coli, Shigella sp., Staphylococcus aureus, antibiotics


Download data is not yet available.


Abiade-Paul, C., Kene, I. & Chah, K. (2005). Occurrence and antibiogram of Salmonellae in effluent from Nsukka Municipal abattoir. Nigerian Veterinary Journal, 27(1), pp.48–53.
Adeyemi, I.G. & Adeyemo, O.K. (2007). Waste management practices at the Bodijaabattoir, Nigeria. International Journal of Environmental Studies, 64(1), pp.71–82.
Adeyemo, O. (2002). Unhygienic operation of a city abattoir in south western Nigeria: environmental implication. African Journal of Environmental Assessment and Management, 4(1), pp.23–28.
Adeyemo, O., Ayodeji, I. & Aika-Raji, C. (2002). The water quality and sanitary conditions in a major abattoir (Bodija) in Ibadan, Nigeria. African Journal of Biomedical Research, 5, pp.51–55.
APHA. (1980). Standard Methods for the Examination of water and Waste Water (19th Ed.) American Public Health Association and water pollution control federation. Washington D. C. pp 1134.
APHA. (1985). Standard methods for the examination of water and wastewater, 16th edition. American Public Health Association, Washington DC.
Bartholomew J. W and Mitter T. (1952).The gram stain. Microbiology and Molecular biology Review. 16, pp.1-29.
Benka-Coker, M. & Ojior, O.(1995). Effect of slaughterhouse wastes on the water quality of Ikpoba River, Nigeria. Bioresource Technology, 52, pp.5–12.
Berger, P.S. & R.K. Oshiro. (2002). Source water protection: Microbiology of source water, pp. 2967-2978, In: Encyclopedia of Environmental Microbiology, G. Bitton, editor in chief, Wiley Inter science, N.Y.
Black, R. E., Cousens, S., Johnson, H. L., Lawn, J. E., Rudan, I., Bassani, D. G.,Jha, P., Campbell, H., Fischer Walker, C., Cibulskis, R., Eisele, T., Liu, L. & Mathers, C. (2010). Global, regional, and national causes of child mortality in 2008: a systematic analysis. Lancet, 375(9730), pp.1969–87.
Blake, D. P., Hillman, K., Fenlon, D. R., & Low, J. C. (2003). Transfer of antibiotic resistance between commensal and pathogenic members of the Enterobacteriaceae under ileal conditions. Journal of Applied Microbiology, 95(3), pp.428–436.
Dufour, A., Bartram, J., Bos R & Gannon V. (2012). Animal Waste, WaterQuality and Human Health. IWA Publishing, London.
Garrity G, Brenner DJ, Krieg NR, Staley JR. (2005). Bergey's manual of systematic bacteriology, vol 2: the proteobacteria, part B: the gammaproteobacteria. Springer, New York, NY, USA.
Krishnaveni, R., Devi, P. Y. and Rao, S. R. (2013). Bioremediation of steel industrial effluents using soil microorganisms.International Journal of Advance Biotechnology and Research. 4(1), pp.51-56.
Magiorakos, A. P., Srinivasan, A., Carey, R. B, Carmeli, Y., Falagas, M. E., Giske, Harbarth C. G., Hindler S., J. F., Kahlmeter, G, Olsson-Liljequist, B., Paterson, D. L., Rice,
L. B., Stelling, J., Struelens, M. J., Vatopoulos, A., Webber, J. T. & Monnet, D. L. (2012). Multidrug ‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection. 8(3), pp.268–281.
Mukonzo, J. K., Namuwenge, P. M., Okure, G., Mwesige, B., Namusisi, O. K., & Mukanga, D. (2013). Over-the-counter suboptimal dispensing of antibiotics in Uganda. Journal of Multidisciplinary Healthcare, 6, pp.303–310.
Nwanta, J.A., Onunkwo, J. & Ezenduka, E. (2010). Analysis of Nsukka metropolitan abattoir solid waste and its bacterial contents in south eastern Nigeria: public health implication. Archives of environmental & occupational health, 65(1), pp.21–6.
Selvi, A. T., Anjugam, E., Devi, R. A.,Madhan, B.,Kannappan, S. and Chandrasekaran, B. (2012).Isolation and Characterization of Bacteria fromTannery Effluent Treatment Plant and their Tolerance to Heavy Metals and Antibiotics.AJEBS, 3(1), pp. 34-41.
Swai, E.S. and Schoonman, L. (2012). A survey of zoonotic diseases in trade cattle slaughtered atTanga city abattoir: a cause of public health concern. Asian Pacific Journal of Tropical Biomedicine, 2(1), pp. 55-60.
United States Environmental Protection Agency. (2006). "Terms of Environment Beginning With"E"". Assessed June 9, 2010.
WHO 2013: World Health Organization. (2013). Guidelines for Drinking-water Quality, 4th ed. (2013), pp. 2
448 Views | 572 Downloads
How to Cite
Bose, D., & Kime, M. (2019). Antimicrobial Susceptibility for Bacterial Isolates from Abattoir Effluent of Damaturu-Nigeria. International Journal of Advancement in Life Sciences Research, 2(2), 27-35. Retrieved from