Effect of Vitamin C & E on Enzyme Glutathione Peroxidase Activity (GSH-Px) in Male Wistar Strain Rats Exposed to Carbon Tetrachloride (CCl4)

  • Titin Ifayanti Lecturer, STIKes Alifah Padang, Indonesia


The free radicals are the cause of cell damage that underlies the emergence of various degenerative diseases. Having an abundant amount of free radicals can cause oxidative stress. Carbon tetrachloride, including chemicals that are metabolized in the liver, its metabolism result is free radical. Its needed to anticipate the damage to the liver. So that, it is needed to have antioxidants from outside to increase the cells defence as neutralizer free radicals like vitamin C and E.

The purpose of this study was to determine the effect of vitamin C and E on glutathione peroxidase enzyme activity on rats exposed to carbon tetrachloride.

The research was conducted experimentally with post test only control group design. The study sample consisted of 25 male wistar strain rats weighing 165-210 g, divided into 5 groups: negative control, positive control (CCl4), treatment I (CCl4 and Vitamin C), treatment II (CCl4  and Vitamin E),  treatment III (CCl4,  combination of vitamins C and E). CCl₄ was given a single dose of 0.2 mg / kg, the next 24 hours are given Vitamin C 0.8 mg / kg, 0.8 ml vitamin E / kg and a combination of vitamins C 0.8 mg / kg and 0.8 ml/ kg, administered orally for 14 days. Data were analyzed with a confidence level of 95%.

The Results of the study showed the average activity of the enzyme glutathione peroxidase was negative group 114.4 ± 5.5 nmo/ml, positive group 96 ± 7.4 nmo/ml, the first treatment 129.2 ± 5.4 nmo/ml, the second treatment  140.2 ± 3.2nmo/ml, the third treatment 156 ± 4.2 nmo/ml.

It is concluded that vitamin C and E can increase the glutathione peroxidase activity in rats exposed to carbon tetrachloride. It is need to do more research on the effect of vitamin C and E to see the existing network of the liver.

Keywords: Carbon Tetrachloride, Glutathione Peroxidase, Vitamin C and E


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Almatsier S. (2009). Basic Principles of Nutritional Sciences. Jakarta. PT. Gramedia Main Library.
ATSDR (Agency for Toxic Substances and Disease Registry) 2005. Toxilogikal profile for carbon tetraclorida. Atlanta GA: US. Department of Public Health and Human Services Public Health Service. Nutritional Biochemistry of the Vitamins. Cambridge University Pres. Ed 2 nd.
Chevion, S. et al, (2003). Plasma antioxidant status and cell injury after severe physical exercise. Proc Nati Acad Sci. 100 (9), pp. 5119-5123.
Cochrane G.C. (1991). Celluler injury by oxidants. Am.J. Med. Department of Immunology, IMM12, Research Institute of Scripps Clinic, North Torrey Pines Road, 92037, La Jolla, CA, USA. Pp. 177-188
Dahiru D, ObiO and Umaru H. (2003). Effect of hibiscus sabdariffa calyx extract on carbon tetrachloride induced liver damage. Biochemistry 15 (1), pp. 27-33
Daruningrum I. (2012). Measurements of GSH levels in rat liver and rat blood. Practicum Report on Oxidative Metabolism and Stressas Techniques. Biomedical Masters Program in Medical Sciences, University of Indonesia. Jakarta.
Ernawati F. (2009). The effect of Vitamin C supplementation is campared with multivitamin mineral on antioxidant nutrisional status in women workers. Faculty of Human Ecology, Bogor Agrycultural University. Indonesian Nutrition Journal.
Farrell G. (2008). Cirrhosis in Asians: Less Common Than In Europeans. Journal of Gastroenterology and Hepatology, 23 (4).
Halliwel B. , Gutteridge J.M. (2004). Free radicals in biology and medicine Oxford.
Hariyatmi, (2004). The abilityof Vitamin E as an antioxidant against free radicals in the enderly. MIPA Journal Surakarta. UMS.14(1).
Lampe J.W. (1999). Health effects of vegetables and fruit: Assessing Mechanism of Action In Human Experimental Studies.
Marks Dawn B., Alan B. Marks, Smith Collen M., (2000). Basic medical biochemistry: a clinical approach. In Dr. Brahm U: Translator. EGC. Jakarta.
Montgomery K. F., Osborn L., Hession C., Tizerd R., Goff D., Vasallo C., et al. (1991). Activation of Endothelial-Leukocyte Adhesion Molecule 1 Gene Transcription. Proc Natl Acad Sci USA. 88, pp. 6523-6527
Ogeturk M., Kus I., Kavakli A., Zararsiz I., Ilhan N., Sasilmaz M. (2004). Effects of melatonin on carbon tetrachloride-induced changes in rat serum. J Physiol Biochem. 60 (3), pp.205-210
Pavlovic V., Cekic S., Rankovic G. & Stoiljkovic N. (2005). Antioxidant and Pro-oxidant Effect of Ascocbic Acid. Acta Medica Medianae. 44 (1): 65-69.
Ramanathan K., Anusuyadevi M., Shila S., Paneerselvam C. (2005). Ascorbic acid andtocopherol as potent modulators of apoptosis on arsenic induced toxicity in rats. Toxicol Lett. 156, pp. 297-306.
Rendon-Remisez A., Cerbon-Solorzano J., Maldonado-Vrga M., Quintanar-Escorza M. A., Calderon-Salinas J.V., (2007). Vitamin E reduces the oxidative damage on aminolevunic dahidratase induced by lead intoxication in rat erythrocytes. Toxicology in vitro. 21, pp. 1121.
Sastroasmoro, Sudigdo, Ismael, Sofyan. (2011). The basics of clinical research methodology. 4th Edition. Jakarta: Sagung Seto.
Tuminah S. (2009). Media Penelit. dan Pengembang. Kesehat. XIX (Suplemen II), pp.S13-S20
Valko, (2006). Free radicals, metals and antioxidants in oxidativestress-induced cancer. J Chem. Biol. Rusia. Edisi 160, pp.1-40.
Winarsi H., (2007). Natural antioxidants and potential free radicals and their applications in health. Yogyakarta. Kanisius.
WHO, (2007). Carbon Tetrachloride Health And Safety Guide. Available from :
Youngson R. (2005). Antioksidan: Manfaat Vitamin C dan E bagi Kesehatan. Terjemahan oleh Susi Purwoko, Jakarta, Penerbit Arcan, 1.
Zulfita, (2014). Effect of rosella extract on Glutathione Peroxidase activity in rats exposed to Carbon Tetrachloride. (Thesis). Padang. Faculty of Medicine, Andalas University.
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Ifayanti, T. (2019). Effect of Vitamin C & E on Enzyme Glutathione Peroxidase Activity (GSH-Px) in Male Wistar Strain Rats Exposed to Carbon Tetrachloride (CCl4). International Journal of Advancement in Life Sciences Research, 2(2), 12-20. Retrieved from https://ijalsr.org/index.php/journal/article/view/54