Assessing The Utility of Genexpert as A Diagnostic Tool in The Detection of Tuberculosis in A Neurological Tertiary Care Setup
Abstract
Tuberculosis in the CNS(central nervous system) is the most dangerous form of extrapulmonary Tuberculosis and is still a formidable clinical challenge. Rapid diagnosis of Tuberculosis and detection of Rifampicin resistance are essential for early disease management. The Xpert/Rif Assay is an automated nucleic acid amplification test that can identify the Mycobacterium tuberculosis DNA and resistance to Rifampicin. In this experiment we used different pulmonary as well as extrapulmonary samples from patients to assess the utility of genexpert as a diagnostic tool.
Keywords:
Genexpert, Tuberculosis, central nervous system, Xpert MTB/Rif assay
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References
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Blakemore, R., Story, E., Helb, D., Kop, J., Banada, P, Owens, M. R., Chakravorty, S., Jones, M., Alland, D. (2010). Evaluation of the analytical performance of the Xpert MTB/RIF assay. J Clin Microbiol. 48(7), 2495-501.
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Helb, D., et al. (2010). Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48, 229–237
Helb, D., Jones, M., Story, E., Boehme, C., Wallace, E., Ho, K., Kop, J., Owens, M. R., Rodgers, R., Banada, P., Safi, H., Blakemore, R., Lan, N. T., Jones-López, E. C., Levi, M., Burday, M., Ayakaka, I., Mugerwa, R. D., McMillan, B., Winn-Deen, E., Christel, L., Dailey, P., Perkins, M. D., Persing, D. H., Alland, D. (2010). Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J Clin Microbiol. 48(1), 229-37.
Lawn, S. D., Mwaba, P., Bates, M., Piatek, A., Alexander, H., et al. (2013). Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test. Lancet Infect Dis 13, 349–361.
Pai, M, Kalantri, S., Dheda, K.. (2006). New tools and emerging technologies for the diagnosis of tuberculosis: part I. Latent tuberculosis. Expert Rev Mol Diagn. 6(3), 413-22.
Traore, H., Fissette, K., Bastian, I., Devleeschouwer, M., Portaels, F. (2000) Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. Int J Tuberc Lung Dis. 4(5), 481-4.
Wallis, R. S., Pai, M., Menzies, D., Doherty, T. M., Walzl, G., Perkins, M. D., Zumla, A. (2010). Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet. 29, 375(9729),1920-37.
WHO Factsheet, (2016). available at www.who.int/tb/publications/factsheet_global.pdf
World Health Organization (2009). Global tuberculosis control. A short update to the 2009 report. Geneva, Switzerland.
World Health Organization.Global tuberculosis control (2010). Publication no. WHO/HTM/2010.7. Geneva, Switzerland.
Behr, M. A., Warren, S. A., Salamon H, Hopewell, P. C., Ponce de Leon, A., Daley, C. L., Small, P. M. (1999). Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli. Lancet. 6, 353(9151), 444-9.
Blakemore, R., Story, E., Helb, D., Kop, J., Banada, P, Owens, M. R., Chakravorty, S., Jones, M., Alland, D. (2010). Evaluation of the analytical performance of the Xpert MTB/RIF assay. J Clin Microbiol. 48(7), 2495-501.
Bryan, R., Michael Olin, Cristina A. Baker, Thomas, Molitor W., Phillip K. Peterson (2008). Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects Clin. Microbiol. Rev. 21(2), 243-261
Cheng, V. C., Yew, W. W., Yuen, K. Y. (2005). Molecular diagnostics in tuberculosis. Eur J Clin Microbiol Infect Dis, 24(11), 711-20.
Cherian, A., Thomas, S. V. (2011) Central Nervous system. Afri health Sci, 11(1), 116–127.
Gupta, P. R. (2004). Difficulties in managing lymph node tuberculosis. Lung India; 21, 50-3.
Helb, D., et al. (2010). Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48, 229–237
Helb, D., Jones, M., Story, E., Boehme, C., Wallace, E., Ho, K., Kop, J., Owens, M. R., Rodgers, R., Banada, P., Safi, H., Blakemore, R., Lan, N. T., Jones-López, E. C., Levi, M., Burday, M., Ayakaka, I., Mugerwa, R. D., McMillan, B., Winn-Deen, E., Christel, L., Dailey, P., Perkins, M. D., Persing, D. H., Alland, D. (2010). Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J Clin Microbiol. 48(1), 229-37.
Lawn, S. D., Mwaba, P., Bates, M., Piatek, A., Alexander, H., et al. (2013). Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test. Lancet Infect Dis 13, 349–361.
Pai, M, Kalantri, S., Dheda, K.. (2006). New tools and emerging technologies for the diagnosis of tuberculosis: part I. Latent tuberculosis. Expert Rev Mol Diagn. 6(3), 413-22.
Traore, H., Fissette, K., Bastian, I., Devleeschouwer, M., Portaels, F. (2000) Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. Int J Tuberc Lung Dis. 4(5), 481-4.
Wallis, R. S., Pai, M., Menzies, D., Doherty, T. M., Walzl, G., Perkins, M. D., Zumla, A. (2010). Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet. 29, 375(9729),1920-37.
WHO Factsheet, (2016). available at www.who.int/tb/publications/factsheet_global.pdf
World Health Organization (2009). Global tuberculosis control. A short update to the 2009 report. Geneva, Switzerland.
World Health Organization.Global tuberculosis control (2010). Publication no. WHO/HTM/2010.7. Geneva, Switzerland.
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How to Cite
Prabal, P., & Maiti, S. (2018). Assessing The Utility of Genexpert as A Diagnostic Tool in The Detection of Tuberculosis in A Neurological Tertiary Care Setup. International Journal of Advancement in Life Sciences Research, 1(3), 22-29. Retrieved from https://ijalsr.org/index.php/journal/article/view/25
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