Phytochemicals: Extraction, Isolation & Identification of Bioactive Compounds from Aristolochia bracteolate

Phytochemicals from Aristolochia bracteolate


Objective:To isolate, analyze and identify the phytochemical compositions from  Aristolochia bracteolate  leaves. Methods: Preliminary phytochemical analysis for four active constituents, extracted  from powdered leaves with different solvents by using Soxhlet apparatus and carried out by different chromatographic techniques. The phytochemical characterizations were evaluated by NMR & MS. Results: This species exhibited & Identified four compounds representing 2.0 % of the total phytochemical composition. The Preliminary phytochemical analysis confirmed the presence of Hydroxystigmast-5-en-7-one, Oleanonic acid, 5,7,4ʹ-trihydroxy-3,8-dimethoxyflavone & 7,9-dimethoxy-tariacuripyrone. All of them are report for first time from this Spp. Conclusions: From this study, can be concluded that report  for the first time from  Aristolochia bracteolate leaves, identified 4 pure chemical compounds, by analysis using various physical & spectral techniques.

Keywords: Aristolochia bracteolate leaves; Hydroxystigmast-5-en-7-one, Oleanonic acid, 5,7,4ʹ-trihydroxy-3,8-dimethoxyflavone, 7,9-dimethoxy-tariacuripyrone, NMR analysis


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Chauhan, N., Kumar, D., & Kasana, M. S. (2009). Medicinal plants of Muzaffarnagar district used in treatment of urinary tract and kidney stones.
Kuo, P. C., Li, Y. C., & Wu, T. S. (2012). Chemical Constituents and Pharmacology of the Aristolochia (馬兜鈴 mădōu ling) species. Journal of traditional and complementary medicine, 2(4), 249-266.
Lopes, L. M. X., Nascimento, I. R., Silva, T. D., & Da, L. M. (2001). Phytochemistry of the Aristolochiaceae family. Research advances in phytochemistry, 2, 19-108.
Manjunath, B. L. (1948). The Wealth of India. A Dictionary of India Raw Material and Industrial Products. Vol. 1. Council of Scientific and Industrial Research, New Delhi, Republic of India.
Rastogi, R. P., & Mehrotra, B. N. (1990). Compendium of Indian medicinal plants. Central Drug Research Institute.
Shirwaikar, A., & Somashekar, A. P. (2003). Antiinflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam. Indian journal of pharmaceutical sciences, 65(1), 67-69.
Suliman Mohamed, M., Timan Idriss, M., Khedr, A. I., Abd AlGadir, H., Takeshita, S., Shah, M. M., ... & Maki, T. (2014). Activity of Aristolochia bracteolata against Moraxella catarrhalis. International journal of bacteriology, 2014.
Tian-Shung, W., Chung-Ren, S., & Ping-Chung, K. (2005). Chemical constituents and pharmacology of Aristolochi species. In Studies in Natural Products Chemistry (Vol. 32, pp. 855-1018). Elsevier.
Tomar, A. (2008). Folk medicinal uses of some indigenous plants of Hastinapur block in Meerut district,(Uttar Pradesh) India. Journal of Medicinal and Aromatic Plant Sciences, 29(4), 186-190.
Tomar, A. (2016). Medicinal use of Abutilon indicum (L.) Sweet (Kanghi) to cure Boil and Ulcer. Journal of Non-Timber Forest Products, 23(3), 157-158
Tomar, A. (2017a). Medicinal use of Aristolochia bracteolata Lam. Journal of Pharmacognosy and Phytochemistry, 6(4), 598-599.
Tomar, A. (2017b). Aerva lanata (Linn.) Juss. Use to cure headache. Journal of Medicinal Plants Studies, 5(2), 329-330.
Tomar, A. (2017c). Folk medicinal use of Blumea lacera (BURM. F.) DC. To cure thread worms. Journal of Medicinal Plants Studies, 5(2), 336-337.
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Nasser, J. (2020). Phytochemicals: Extraction, Isolation & Identification of Bioactive Compounds from Aristolochia bracteolate. International Journal of Advancement in Life Sciences Research, 3(3), 50-54. Retrieved from
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