Amplification and sequence analysis of ‘nad1’ gene from edible mushroom Lentinula edodes

Analysis of fungal nad1 gene.

  • Tanushree Paul Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398 Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata 700125, India https://orcid.org/0000-0002-0850-6183
  • Shreyoshi Pal Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398 Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata 700125, India https://orcid.org/0000-0003-0851-3233
  • Dipanwita Roy Division of Plant Biology, Bose Institute, P1/12, C.I.T., Scheme VII M, Kakurgachhi, Kolkata 700054, India
  • Samir Ranjan Sikdar Division of Plant Biology, Bose Institute, P1/12, C.I.T., Scheme VII M, Kakurgachhi, Kolkata 700054, India
  • Pijush Mallick Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398 Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata 700125, India & Division of Plant Biology, Bose Institute, P1/12, C.I.T., Scheme VII M, Kakurgachhi, Kolkata 700054, India https://orcid.org/0000-0002-4531-7744

Abstract

Edible mushrooms are being used as food supplement worldwide for its nutritional attributes. Crop improvement through molecular breeding is adopted in mushroom for different significant targets as well. Thus the molecular genetic analysis is prerequisite and had been carried out for last few decades. Lentinula edodes is a popular shiitake mushroom having medicinal properties and high volume of food value and consume in mainly Asian countries. Genetic studies of L. edodes already been done due to its popularity in breeding programme too. Here, the NADH complex subunit is chosen first time in mushroom to standardize its amplification conditions and sequence analysis. Through the nad1 gene is a conserved region, we tried to give an account that it can be used as genetic marker in various studies in future. However, we have successfully standardized the PCR conditions and showed the nucleotide similarity of L. edodes with its mitochondrial genome. The amplified gene showed 378 bp in size and 99% similarity in this study.

Keywords: Edible mushroom, Lentinula edodes, ‘nad1’ gene, BLAST

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