Effect of Sambiloto (Andrographis paniculata) Leaves against Cholesterol Levels in Wistar Rats with Atherogenic Diet


Introduction : The risk of atherosclerosis increases when elevated blood cholesterol levels. Flavonoids may help inhibit the absorption of fat, which indirectly helps in lower cholesterol levels.Flavonoids are widely available on the Andrographis paniculata leaves (APL).This study aims to examine the effect of flavonoids in APL stewto lower the cholesterol levels. Material and Methods: This research was carried out using five different groups of Wistarrats. Each group consists of five Wistar rats. Group (K-) received normal diet, group (K+) received atherogenic diet and three groups of atherogenic diets rats that pre-treated with three different doses (0.6, 1.2, and 2.4g/day) of APL leaf stew. APL stew is orally gavaged for 60 days. The cholesterol blood serum was analyzed using the CHOD-PAP method.  Statistical analysis using One-Way ANOVA and Post-Hoc Tukey. Results : The results showed a significant difference in cholesterol levels between the groups of theatherogenic diet with other treatment groups (p = 0.000).When compared with thenormal diet group, treatment with 1.2 g and 2.4 g of APL produce cholesterol levels not significantly different.APLstew has been shown to inhibit the elevated levels of serum cholesterol in male Wistar rats that fed with the atherogenic diet which contributed 54.8%.While fat intake increased the cholesterol level by 16.3%.Both APL stew and fat dose intake together affect the formation of cholesterol with a contribution of 69.2%. Conclusion : The dose that is considered as the most effective in lowering cholesterol is 2.4 g becauseit producescholesterol level closest to normal.

Keywords: Andrographis paniculata; atherogenic diet; cholesterol


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Agrawal, R.C. and Pandey, P., 2019. Screening of AndrographisPaniculata Extract For Antioxidant And Genotoxic Activities. International Journal of Research-GRANTHAALAYAH, 7(6), Pp.132-142.
Bharati, B.D., Sharma, P.K., Kumar, N., Dudhe, R. And Bansal, V., 2011. Pharmacological Activity OfAndrographisPaniculata: A Brief Review. Pharmacology online, 2(1), P.10.
Dai, Y., Chen, S.R., Chai, L., Zhao, J., Wang, Y. And Wang, Y., 2019. Overview Of Pharmacological Activities of Andrographis paniculata and Its Major Compound Andrographolide. Critical Reviews in Food Science and Nutrition, 59(Sup1), Pp.S17-S29.
Hariadini, A.L., 2020. Hubungan Tingkat Pengetahuan Dan Ketepatan Penggunaan Obat Simvastatin Pada Pasien Hiperkolesterolemia Di Apotek Kota Malang. Pharmaceutical Journal OfIndonesia, 5(2), Pp.91-96.
KKRI (2019) http://p2ptm.kemkes.go.id/kegiatan-p2ptm/pusat-/hari-jantung-sedunia-hjs-tahun-2019-jantung-sehat-sdm-unggul. Accessed at 05/29/2021
Lakshmia, V., Srivastav, S., Khanna, A.K., Mahdi, A.A. And Agarwal, S.K., 2014. Lipid Lowering Potential of Andrographis paniculata (Nees). The Journal of Phytopharmacology, 3(2), Pp.124-129.
Liu, Y.T., Chen, H.W., Lii, C.K., Jhuang, J.H., Huang, C.S., Li, M.L. And Yao, H.T., 2020. A Diterpenoid, 14-Deoxy-11, 12-Didehydroandrographolide, In AndrographisPaniculata Reduces Steatohepatitis And Liver Injury In Rat Fed A High-Fat And High-Cholesterol Diet. Nutrients, 12(2), P.523.
Mustafa, R.A., Hamid, A.A., Mohamed, S. And Bakar, F.A., 2010. Total Phenolic Compounds, Flavonoids, And Radical Scavenging Activity Of 21 Selected Tropical Plants. Journal of Food Science, 75(1), Pp.C28-C35.
Nugroho, A.E., Andrie, M., Warditiani, N.K., Siswanto, E., Pramono, S. And Lukitaningsih, E., 2012. Antidiabetic AndAntihiperlipidemic Effect of AndrographisPaniculata (Burm. F.) NeesAndAndrographolide In High-Fructose-Fat-Fed Rats. Indian Journal of Pharmacology, 44(3), P.377.
Polash, S.A., Saha, T., Hossain, M.S. And Sarker, S.R., 2017. Investigation of The Phytochemicals, Antioxidant, And Antimicrobial Activity of The AndrographisPaniculata Leaf And Stem Extracts. Advances InBioscience And Biotechnology, 8(05), P.149.
Rachmani, E.P.N., 2019. Aktivitas hipoglikemik Dan Hipolipidemik Fraksi Flavonoid HerbaSambiloto (Andrographis paniculata (Burm. F.) Ness.): Efek In Vivo PadaTikus Yang Diinduksi Fruktosa Dan Lemak Tinggi Serta Kajian In Vitro PadaEnzim Alfa-Amilase, Alfa-Glukosidase Dan Antioksidan (Doctoral Dissertation, Universitas Gadjah Mada).
Soltani, S., Boozari, M., Cicero, A.F., Jamialahmadi, T. And Sahebkar, A., 2021. Effects of Phytochemicals on Macrophage Cholesterol Efflux Capacity: Impact on Atherosclerosis. Phytotherapy Research.
Sudarmi, M., Darmini, W. And Wartini, M., 2018, October. Activity OfSambiloto Extract (AndrographisPaniculataNess) In Reducing Total Blood Cholesterol Levels Hypercholesterolemia Rats. In International Conference On Applied Science And Engineering (ICASE 2018) (Pp. 120-122). Atlantis Press.
Violi, F., Pignatelli, P. And Pulcinelli, F.M., 2002. Synergism Among Flavonoids In Inhibiting Platelet Aggregation And H. Circulation, 105, P.E53.
Warditiani, N.K., Sari, P.M.N.A., Ramona, Y. And Wirasuta, M.A.G., 2020. Molecular Pharmacology Study of Andrographolide Extracted From Andrographis paniculata on Atherosclerosis Preventive Effect. Systematic Reviews In Pharmacy, 11(9), Pp.201-206.
Widjajakusuma, E.C., Jonosewojo, A., Hendriati, L., Wijaya, S., Surjadhana, A., Sastrowardoyo, W., Monita, N., Muna, N.M., Fajarwati, R.P., Ervina, M. And Esar, S.Y., 2019. Phytochemical Screening And Preliminary Clinical Trials of The Aqueous Extract Mixture of Andrographis paniculata (Burm. F.) Wall. Ex Nees And Syzygium polyanthum (Wight.) Walp Leaves In Metformin Treated Patients With Type 2 Diabetes. Phytomedicine, 55, Pp.137-147.
Widyani, R., 2019. Potency of Herbal Plants Formulation As Anticholesterole Agent: In Vitro Studies. Systematic Reviews in Pharmacy, 10(2), Pp.253-258.
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Laili, R., Alristina, A., Ethasari, R., & Hayudanti, D. (2021). Effect of Sambiloto (Andrographis paniculata) Leaves against Cholesterol Levels in Wistar Rats with Atherogenic Diet. International Journal of Advancement in Life Sciences Research, 4(3), 26-31. https://doi.org/https://doi.org/10.31632/ijalsr.2021.v04i03.004