Bioefficacy of New Species of Entomopathogenic Nematode, Steinernema Dharanaii (Nematoda: Rhabditida: Steinernematidae) against Whitegrub, Holotrichia Rustica (Coleoptera: Scarabaeidae)

  • Sanjay Dattatray Paunikar Northern Regional Centre, Zoological Survey of India,Deharadun-248 195, Uttarakhand, India & Forest Entomology Division, Tropical Forest Research Institute,P. O. RFRC,Jabalpur- 482021, Madhya Pradesh, India
  • Nitin Kulkarni Director, Institute of Forest Productivity, (IFP) Gumla, Lalgutwa, Ranchi - 835303. Jharkhand, India


The bioefficacy study of new native species of entomopathogenic nematode, Steinernema dharanaii (TFRIEPN-15) against root grubs Holotrichia rustica infecting Tectona grandis crop of forest nurseries of central India was taken up under controlled laboratory conditions. The grub of H. rustica when exposed to range of Infective Juveniles (IJs) numbers dose dependant relationship by 10-15- and 25-30-days old grubs. The younger grubs were more susceptible as compared to older ones. Minimum number of IJs, i.e. 300 Grub-1 causes 13.33% mortality in younger 6 days after the exposure, followed by 46.66% at the IJs population of 600 IJs Grub-1 mortality in older grubs at population of 600 IJs Grub-1 causes 26.66% mortality. IJs population of 900 to 3000 caused mortality ranging from 73.33 to 93.33% in younger grubs at par with each other (P>0.05). IJ population of above 6000 IJs caused 100.0% mortality in younger grub which was significantly superior (P<0.05). The older grub also exhibited similar trends, however maximum of 93.33% mortality at the highest IJ population.
These experiments have proved that indigenous species of entomopathogenic nematodes can be used successfully control white grubs and other soil insect pests with eco-friendly and effective management techniques achieving cent-percent mortality of the target insect pests.

Keywords: Entomopathogenic nematodes, Biological control, Steinernema dharanaii, White grubs, Holotrichia rustica, Forest insect pests


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Abbott, W.S. (1925). A method for computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265–267.
Anitha, V., Rogers, D.J, Wightman. J. & Ward, A. (2006). Distribution and abundance of whitegrubs (Coleoptera: Scarabaeidae) on groundnut in southern India. Crop Protection, 25:732-740.
Ansari, M.A.,Phan, K.L. & Moens M. (2003)..Heterorhabditis bacteriophora (Heterorhabditidae: Rhabditida) parasitic in natural population of white grubs (Coleoptera: Scarabaeidae) in Belgium. Russian Journal of Nematology. 11: 57-59.
Askary, T.H. & Ahmad, M.J. (2020). Efficacy of entomopathogenic nematodes against the cabbage butterfly (Pieris brassicae (L.) (Lepidoptera: Pieridae) infesting cabbage under field conditions. Egyptian Journal of Biological Pest Control. 30, 39.
Bedding. R. (2006).Entomopathogenic nematodes from discovery to application. Biopesticides International. 2: 87-119.
Bedding, R.A. & Akhurst, R.J. (1975). A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica, 21: 109-116.
Beeson, C.F.C. (1941) The ecology and control of forest insects of India and neighboring countries. Vasant Press, Dehradun, India. p: 1007.
Bhatnagar, A. Shinde. V. & Bareth, S.S. (2004). Evaluation of entomopathogenic nematodes against white grub, Maladera insanabilis Brenske International Journal of Pest Management, 50 (4): 285-289.
Bhawane, G.P., Mamlayya A.B., Wagh S.R. & Chougule A.K. (2012). Diversity of white grub beetles and their host range from northern, western ghats, Kolhapur District (MS) India. The Bioscan, 7: 589-596.
Browne. F.G. (1968). Pests and diseases of forest plantation trees. Clerendon Press, Oxford, UK.
Dhaliwal, G.S., Singh, R. & Jindal V.A. (2013). Textbook of Integrated Pest Management. Kalyani Publishers, New Delhi, India. pp: 448.
Divya, K. & Sankar, M. (2009. Entomopathogenic nematodes in pest management. Indian Journal of Science and Technology. 2 (7): 53-60.
Garg, V.K., Kulkarni, N. & Meshram, P.B. (2005). Influence of weather parameters on the incidenceof white grub, Holotrichia serrata Fabr. on teak seedlings in Chhindwara Forest Divisions. Indian Journal of Tropical Biodiversity, 13:48-51.
Gomez, K.A. & Gomez, A.A. (1984). Statistical Procedures for Agricultural Research (2nd ed.), A Wiley-Interscience Publication, John Wiley & Sons, New York. pp: 680.
Grewal, P.S., Koppenhofer, A.M. & Choo H.Y. (2005). Lawn, turfgrass and pasture applications. In: Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI Publishing, Wallingford. Pp: 115–146.
Grewal, P.S., Grewal, S.K., Malik, V.S. & Klein, M.G. (2002). Differences in susceptibility of introduced and native white grub species to entomopathogenic nematodes from various geographic localities. Biological Control, 24: 230-237.
Guo, W., Yan, X., Zhao, G., Chen. J. & Han, R. (2015). Efficacy of entomopathogenic Steinernema and Heterorhabditis nematodes against Holotrichia oblita. Journal of Pest Science. 88:359–368.
Hazir S, Kaya HK, Stock SP & Keskün N (2003). Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turkish J Biology 27:181-202.
Hussaini SS, Rabindra RJ & Nagesh M (2003). Current Status of Research on Entomopathogenic Nematodes in India. Project Directorate of Biological Control, PDBC, Bangalore, India. Pp:218.
Hussaini SS, Nagesh M, Dar M. and Rajeshwari R (2005). Field evaluation of entomopathogenic nemaotdes against white grubs (Coleoptera: Scarabaeidae) on turf grass in Srinagar. Journal of Plant Protection Sciences. 13(1): 190-193.
Jackson, T.A. (1992). Scarabs-pest of the past or the future. In T. A. Jackson and T. R. Glare (eds.), Use of pathogens in scarab pest management. Intercept Ltd., Andover, United Kingdom. Pp:1-10.
Joshi, K.C. & Shukla, P.K. (2001). Recent Trends in Insect Pest Control to Enhance Forest Productivity, Proceedings of a Workshop on Entomology and Biological Control, Sept. 25th, Tropical Forest Research Institute, Jabalpur.
Joshi, K.C. & Meshram, P.B. (2008). Whitegrub, Holotrichia threatening bamboo seedlings and its management. Indian Forester. 134:1257-1260.
Karunakar, G., Easwaramoorthy, S. & David, H. (2000a). Host parasitic interactions between two species of whitegrubs infesting sugarcane and two species of EPNs. Sugar Technology. 2: 12-16.
Karunakaran, G., Easwaramoorthy, S. & David, H. (2000b). Pathogenicity of steinenematida and heterorhabditid nematodes to white grubs infesting sugarcane in India. International Journal of Nematology. 10: 19-26.
Kaya, H.K. (1990). Soil ecology, in Entomopathogenic nematodes in biological control (Gaugler, R. and Kaya, H.K. eds.) CRC Press, Boca Raton, FL. Pp: 93-115.
Kaya, H.K. & Gaugler, R. (1993) Entomopathogenic nematodes. Annual Review of Entomology. 38: 181–206.
Khatri-Chhetri, H.B., Timsina, G.P., Manandhar, H.K. & Moens, M. (2011). Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae). Journal of Pest Science. 84:457–469.
Koppenhofer, A.M. & Fuzy E.M. (2008). Early timing and new combinations to increase the efficacy of neonicotinoid–entomopathogenic nematode (Rhabditida: Heterorhabditidae) combinations against white grubs (Coleoptera: Scarabaeidae). Pest Management Science. 64:725–735.
Koppenhöfer, A.M. & Grewal, P.S. (2005). Compatibility and interactions with agrochemicals and other biocontrol agents. In: Nematodes as Biocontrol Agents. CABI, New York, NY, pp:363-381.
Kulkarni, N. (2010). Bioecology and management of white grub complex in teak forest nursery in India. In Cram, M. (Ed.) Proceedings of the 7th Meeting of IUFRO Working Party 7.03.04 Diseases and Insects in Forest Nurseries, Hilo, Hawaii, USA, July 13to 17, 2009.On-linepublication source: http:// foresthealth / publications/ nursery/I UFRO_7_03_04_HiloProc.pdf , Pp. 84-91.
Kulkarni N., Chandra, K., Wagh, P.N, Joshi, K.C. & Singh, R.B. (2007). Incidence and management of white grub, Schizonycha ruficollis on seedlings of teak (Tectona grandis Linn. f.). Insect Science. 14, 411-418.
Kulkarni, N, Paunikar S, Joshi KC and Rogers J (2009) White grubs, Holotrichia rustica and Holotrichia mucida (Coleoptera: Scarabaeidae) as pests of teak (Tectona grandis L.f.) seedlings. Insect Science. 16:519-525.
Kulkarni, N., Paunikar, S. & Singh, R.B. (2012a). Efficacy and field persistence of some insecticides against the white grub beetle, Holotrichia rustica (Burm.) (Scarabaeidae:Melolonthinae). Indian Journal of Entomology. 74(2): 105-107.
Kulkarni N. (2014). Status of potential of biocontrol component for integrated management of forest insect pests in India. In Biopesticides in Sustainable Agriculture: Progress and Potential, O. Koul, G.S. Dhaliwal, S. Khokar, and R. Singh, eds.), Science Publisher, New Delhi, India, 389-419.
Kulkarni N. (2017). Integrated insect pest management in tropical forestry. Rapisarda, C and Cocuzza, G.E.M. (eds.). Integrated pest management in tropical regions, CABI, UK. pp: 313-342.
Kulkarni, N., Paunikar S., Hussaini. S.S. & Joshi, K.C. (2008). Entomopathogenic Nematodes in insect pest management of forestry and plantations crops: An appraisal. Indian Journal Tropical Biodiversity. 16 (2): 156-165.
Kulkarni N., Paunikar, S., Mishra, V.K. & Daksh, S. (2013). Tolerance of Entomophagenic nematode, Steinernema carpocapsae to some modern insecticides and biopesticides. Annals of Entomology. 31: 129-134.
Kulkarni N, Paunikar S. & Mishra V (2011a). Pathogenicity of entomopathogenic nematode, Heterorhabditis indica Poinar against the defoliator, Spirama retorta Cramer (Lepidoptera: Noctuidae) Insect Pest Management, A current Scenario, 2011. (Eds. Dunston P. Ambrose), Entomology Research Unit, St. Xavier’s College Palayamkottai India. Pp- 312-115.
Kulkarni, N., Paunikar, S., Hussaini, S.S., & Joshi K.C. (2011b). Susceptibility of teak skeletonizer, Eutectona machaeralis Walker to EPN, Heterorhabditis indica Poinar. World J Zoology.6 (1):33-39.
Kulkarni, N., Mishra, V.K. & Paunikar, S.D. (2017). Infectivity of native populations of entomopathogenic nematodes against teak defoliators. Journal of Entomology and Zoology Studies. 5 (6): 639-643.
Kulkarni N., Rizvi A, Vikas Kumar, Paunikar S & Mishra V (2012b). Morphological and molecular characterization of Steinernema dharnaii sp. N. (Nematoda: Steinernematidae): An Entomopathogenic Nematode from India. Indian Journal of Tropical Biodiversity. 20 (2):107-116.
Kulkarni, N., Kushwaha DK, Mishra V.K. & Paunikar, S. (2012c). Effect of economical modification in artificial diet of greater wax moth Galleria mellonella (Lepidoptera: Pyralidae). Indian Journal of Entomology. 74(4):369-374.
Kushida, T., Mamiya, Y. & Mitsuhashi, J. (1987). Pathogenicity of newly detected Steinernema sp. (Nematoda) to scarabaeid larvae injurious to tree seedlings. Japanese. Journal of Applied Entomology and Zoology. 31: 144–149.
Lacey, L.A. & Georgis, R. (2012). Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. Journal of Nematology.44(2):218-225.
Lamani, N., Guledagudda, S.S. & Kambrekar, D.N.(2017). An economic impact of white grub infestation on sugarcane in Northern Karnataka. Journal of Entomology and Zoology Studies. 5(3): 18-23.
Mehta, P.K., Chandel, R.S. & Mathur, Y.S. (2010). Status of whitegrubs in north western Himalaya. Journal of Insect Science. 23:1–14.
Naik, B.G., Maheshwarappa, H.P., Rajkumar M., Kalleshwaraswamy, C.M., Gowdra, N. & Latha, S. (2019). Evaluation of entomopathogenic nematodes for the management of white grub, Leucopholis lepidophora Blanchard (Coleoptera: Scarabaeidae). Journal of Entomology and Zoology Studies. 7(1): 09-13.
Nair, K.S. S (2007). Tropical Forest Insect Pests: Ecology, Impact and Management. Cambridge University Press, New York.
Oka, A.G. & Vaishanpayan, S.M. (1979). White grubs menace in teak nurseries in Maharashtra. Second All India Symposium on Biology and Ecology. April, 1979. Bangalore, India.
Paschapur, A.U., Vijaya Lakshmi, K., Sunanda, B.S. & Pawar, V. (2017). Virulence of Entomopathogenic Nematode (Heterorhabditis indica) against Sugarcane root grub (Holotrichia consanguinea). Bulletin Environment Pharmacology and Life Science. 6(1): pp 97-103.
Patil, J. & Rangasamy, V. (2018). Field evaluation of the entomopathogenic nematodes against the white grub, Leucopholis lepidophora Blanchard (Coleoptera: Scarabaeidae). Egyptian Journal of Biological Pest Control.28:41.
Paunikar, S.D. (2014). Bioefficacy of entomopathogenic nematode native to Madhya Pradesh for the management of major forest insect pests. Ph.D. Thesis, Rani Durgawati University, Jabalpur Madhya Pradesh, India.
Paunikar, S. & Kulkarni, N. (2019a). Evaluation of new species of entomopathogenic nematode, Steinernema dharanaii, (TFRIEPN-15) against Bamboo leaf roller, Crypsipyta coclesalis Walker (Lepidoptera: Pyralidae) in the laboratory. Indian Forester. 145(8):767-773.
Paunikar, S. & Kulkarni, N. (2019b) Bioefficacy and Progeny Production of native new-to-science species of entomopathogenic nematodes, Steinernema dharanaii (TFRIEPN-15) against forest insect pest, Albizia defoliator, Spirama retorta Cramer (Lepidoptera: Noctuidae). Research Journal of Agriculture and Forestry Science. 7(4):10-16.
Paunikar, S. & Kulkarni, N. (2019c). Efficacy of Entomopathogenic nematode, Steinernema dharanaii (TFRI-EPN-15) against termites, Odontotermes obesus (Isoptera-Termitidae) in the laboratory. Indian Journal of Forestry. 42 (4): 105-108.
Paunikarm S., Mishra, V., Kulkarni, N. & Hussaini, S.S. (2012). Tolerance of EPN, Heterorhbditis indica to some biopesticides. Pestology. XXXVI (3):41-44.
Paunikar, S., Mishra, V., Bhandari, R. & Kulkarni N. (2010a). Entomopathogenic nematodes as biological control agents in insect pests management. Vaniki Sandesh. 1 (4): 11-17
Paunikar, S., Kulkarni, N., Mishra, V., Tiple, A., & Hussaini, S.S. (2010b). Susceptibility of Teak Skeletonizer, Eutectona machaeralis Walker to Steinernema carpocapsae, Hislopia Journal. 1 (2):165-170.
Paunikar, S., & Kulkarni, N. (2020a). Compatibility of new species of entomopathogenic nematode, Steinernema dharanaii Kulkarni et al., 2012 (Nematoda: Rhabditida: Steinernematidae) from India with some modern biopesticides. International Journal of Environment, Agriculture and Biotechnology, 5(3) :99-105.
Paunikar, S. & Kulkarni, N. (2020b). Pathogenicity and progeny production of new species of entomopathogenic nematode, Steinernema dharanaii Kulkarni et al., 2012 (Nematoda: Steinernematidae) against teak skeletonizer, Eutectona machaeralis Walker (Lepidoptera: Pyralidae) Walker under laboratory condition. International Journal of Zoology and Applied Biosciences. 5(3):170-170.
Paunikar, S. & Kulkarni, N. (2018). of Entomopathogenic nematode, Steinernema dharanaii n.r. (TFRI-EPN-15) on greater wax moth, Galleria mellonella. Indian Journal of Entomology. 80 (4): 1506-1512.
Rajeswari, S., Menon, P.P. & Sivagami, V. (1984). Preliminary tests with DD-136 for the control of potato chafer grub, Anomala sp. Indian Journal of Nematology. 14: 187-188.
Rathee, M. & Dalal, P. (2018). Emerging Insect Pests in Indian Agriculture. Indian Journal of Entomology, 80(2): 267-281.DOI No.: 10.5958/0974-8172.2018.00043.3.
Sankaranarayanan, C. & Askary, T.H. (2017). Status of entomopathogenic nematodes in integrated pest management strategies in India. (eds. M.M.M. Abd-Elgawad, T.H. Askary and J. Coupland). CAB International. pp :362-382.
Sankaranarayanan, C., Somasekar, N. & Singaravelu B. (2006). Biocontrol Potential of Entomopathogenic Nematodes Heterorhabditis and Steinernema against Pupae and Adults of White grub Holotrichia serrata Fab. Sugar Technology.8 (4): 268-271.
Shapiro-Ilan, D.I., Han, R. & Qiu X. (2014). Production of entomopathogenic nematodes. In Mass Production of Beneficial Organisms, Invertebrates and Entomopathogens (Ramos, J.M., Rojas, M.G., Shapiro-Ilan, D.I. eds.), Elsevier Inc., USA. Pp: 321-355.
Sharma, A., DeshRaj & Chandla V.K. (2009). Use of Steinernema and Heterorhabditis nematodes for control of white grubs, Brahmina coriacea hope (Coleoptera: Scarabaeidae) in potato crop. Potato Journal. 36 (3-4): 160-165.
Sharmila, R., Subramanian, S., Poornima, K. & Anitha, K. (2019). Potential of Biocontrol Efficacy of Entomopathogenic Nematodes on White Grubs, Anomala communis (Coleoptera: Scarabaeidae) in Potato. International Journal of Research Studies in Zoology. 5(4): 8-15
Singh, A. & Upadhyay, V. (2018). A Review on Entomopathogenic Nematodes: Heterorhabditis and Steinernema. Advances in Bioresearch. 9 (2): 214-222.
Supekar, S. & Mohite, P. (2015). Utilization of Entomopathogenic nematodes against white grub Holotrichia serrata Fab. infecting sugarcane. Journal of Global Biosciences, 4 (8):3178-3181.
Thakare, V.G. & Zade V.S. (2012). Diversity of beetles (Insecta: Coleoptera) from the vicinity of Semadoh Makhala road, Sipnarange, Melghat Tiger Reserve, (MS) India. Bioscience Discovery. 3: 112-115.
Thakur, M.L. (2.000). Forest Entomology. Sai Publishers, Dehradun. p: 609.
Theurkar, S.V., Patil, S.B., Ghadage, M.K., Zaware, Y.B. & Madan, S.S. (2013). Distribution and abundance of White grubs (Coleoptera: Scarabaeidae) in Khed Taluka, part of Northern Western Ghats, MS, India. International Research Journal of Biological Science. 1(7): 58-60.
Vashisth, S., Chandel, Y.S, & Chandel R.S. (2018). Biological control potential of North West Himalayan strains of Heterorhabditid nematodes against the turnip moth, Agrotis segetum (Denis & Schiffermuller) (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control. 28:37.
Vega, F.E. & Kaya, H.K. (2012). Preface. In:(Eds.). Insect Pathology, Seconded. Academic Press, SanDiego. p.xiii.
Yadav, C.P.S & Sharma, G.K. (1995). Indian white grubs and their management. All India Coordinated Research Project on white grubs, Technical Bulletin No. 2, ICAR, New Delhi, India.
Yadav, I. C. & Devi N.L. (2017). Pesticides Classification and Its Impact on Human and Environment. Environment Science and Engineering. 6: 141-157.
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Paunikar, S., & Kulkarni, N. (2020). Bioefficacy of New Species of Entomopathogenic Nematode, Steinernema Dharanaii (Nematoda: Rhabditida: Steinernematidae) against Whitegrub, Holotrichia Rustica (Coleoptera: Scarabaeidae). International Journal of Advancement in Life Sciences Research, 3(3), 20-29. Retrieved from
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